Blots were processed with ECL Plus Western Blotting detection kit and the signal detected using an LAS-3000 imanalyzeryzer (Fuji Photo Film Co., Tokyo, Japan).
After blotting with respective antibodies, blots were processed with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA) and exposed to BioBlot BXR film (Laboratory Products Sales , Inc Rochester, NY, USA).
All the blots were processed with ECL Plus Western Blotting detection kit (GE Healthcare, Piscataway, NJ, USA), and the signal was detected by a Typhoon 9410 Variable Mode Imager.
Western blotting onto nitrocellulose (0.45 μm, Schleicher and Schuell) was performed according to standard protocols and blots were processed with a chemiluminescence system (ECL, Amersham).
Blots were processed with Enhanced chemiluminescence (ECL) plus Western blotting detection kit and the signal detected using an LAS-3000 imanalyzeryzer (Fuji Photo Film Co).
The blots were processed with ECL Chemiluminescence kit (Amersham Biosciences), and the signal was detected by exposing the processed blots to X-ray films (Biomax Films, Kodak, NY, USA).
The blots were processed with ECL chemiluminescence kit (Amersham Biosciences, Piscataway, NJ, USA), and the signal was detected by exposing the processed blots to X-ray films (Biomax Films, Kodak, NY, USA).
The probed blot was processed in a phosphorimager (Storm; GE Healthcare).
Blot image files were processed with MultiGauge (FujiFilm Lifescience).
Blots were processed and incubated with antibodies as described above.
