Sentence examples for blot was prepared using from inspiring English sources

For the control membrane, a similar blot was prepared using biotinylated soybean trypsin inhibitor (Fluorokine® kit).

A western blot was prepared using the nuclear and cytosolic extracts and probed for the nuclear proteins histone H3 and lamin A/C.

Northern blot was prepared using 20 μg total RNA and probes were prepared by labeling the PCR amplicons with αP-dATP using HexaLabel DNA labeling kit (Fermentas Life Sciences, USA).

A small RNA blot was prepared using a 15% denaturing gel, consisting of 15 ml SequaFlowGel sequencing system concentrate, 7.5 ml SequaFlowGel diluent, 2.5 ml 10× MOPS buffer, 250 μl 10% ammonium persulfate (Sigma, Poole, Dorset, UK) and 25 μl tetramethylethylenediamine.

According to the protein concentrations, samples for western blot were prepared using the 4× NuPage LDS sample buffer (Invitrogen , Inc.

Cell lysates for western blot were prepared using SDS-buffer (62.5 mℳ Tris-EDTA, pH6.8, 5% SDS) and total protein content was measured using the BCA protein assay kit (Bio-Rad Laboratories, Hertfordshire, UK).

Total RNA for Northern blots was prepared using Qiagen RNeasy Midi kits.

The probe for detection of the tet(L) gene after Southern blotting was prepared using the same PCR amplicon, which was labeled using the ECL direct nucleic acid labeling and detection kit (GE Healthcare, HCMC, Vietnam).

Higher quality tissue DNA, used for Southern blotting, was prepared using the Puregene® DNA Purification Kit (Gentra Systems, Minneapolis, MN, USA): 10 20 mg tissue was crushed in liquid nitrogen with a pestle and mortar and the DNA extracted according to the manufacturer's instructions.

In complementary experiments, Western blots were prepared using control tissue obtained from an adult rat-brain microsomal preparation or the total lysate from the SVZ eNPC (Figure 2 C).

Blots were prepared using antibodies as described [23].