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Western blot was performed using proteins extracted with RIPA buffer (50 mM Tris-HCl, pH7.4, 150 mM NaCl, 1 mM dithiothreitol, 1% Triton X-100, 1% sodium deoxycholate).
Western blot was performed using the methods mentioned above.
Western blot was performed using previously described procedures [45].
Southern blot was performed using DIG DNA Labeling and Detection Kit (Roche) according to the manufacturer's protocol.
Western blot was performed using an XCell II blot module (Invitrogen, San Diego, CA) and a Protran nitrocellulose membrane (Schleicher & Schuell, Dassel, Germany) with 0.1 mm pore size.
Total RNA was isolated using TRIZOL Reagent (Invitrogen), and northern blot was performed using Random Primer DNA Labeling Kit (TaKaRa) and analyzed by BAS2500 (Fuji Film).
Similar(9)
The dot blotting was performed using streptavidin-HRP.
Western blotting was performed using anti-Pfs25 4B7 mAb.
Western blotting was performed using total histones equivalent to 0.5 μg of genomic DNA.
Anti-Rac1 antibodies were used to precipitate the cell lysates, and western blotting was performed using HA-specific antibodies.
Samples were resolved on a 12% gel and western blotting was performed using anti-LDHA and anti-LDHB antibodies.
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