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Final imaging of the blot was done using the Fluor Chem Q system (Protein Simple, Santa Clara, CA).
The proteins were separated by SDS-PAGE and the western blot was done using p27 and CDK 4 antibody.
Western blot was done using rabbit anti-phospho[Y]-SFK antibody (1 : 1000; Cell Signaling Technology) to detect phosphorylated lyn and src.
Ten microlitres from each elution fraction, along with proper controls, was resolved by SDS PAGE gel, and Western blot was done using a goat anti-human Fc IgG1-HRP-conjugated antibody.
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Western blot were done using 30 µg of nuclear extracts and according to Perilhou et al. [7].
Northern blotting was done using standard techniques.
Northern blotting was done using NorthernMax formaldehyde-based system (Ambion Inc., Austin, TX) according to the manufacturer's recommendations.
Densitometric analysis of Western blots was done using the Kodak Gel Logic system.
Quantification of western blots was done using Multi-Gauge (Fuji-Film) and Image J (NIH).
Densitometric analysis of western blots was done using AlphaEase FC software (Alpha Innotech, Santa Clara, CA).
Analysis of the western blots was done using the Image J software (National Institute of Mental Health; http://rsb.info.nih.gov/ij).
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