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Intensity of the blot was determined using the Image J software.
Intensity of the blot was determined using the Image J software (NIH, Bethesda, MD, USA).
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Immunodetection of the protein blotted was determined using a Fujifilm Intelligent Dark Box II.
Relative band density for Western blotting was determined using ImageJ gel analysis software.
The intensity of the bands in stained gels and blots was determined using Nonlinear.
Density of bands on film exposed to blots was determined using Scion Image software (Scion Corp., Frederick, MD, USA).
Statistical significance in glucose uptake assays, glycogen synthesis assays, and Western blotting was determined using RM two-way ANOVA followed by the Bonferroni multiple comparison test.
After incubation with second antibody (1 : 2500) for 1 h at room temperature, the blots were determined using an ECL Western blotting kit (Amersham Pharmacia Biotech, Buckinghamshire, UK), according to the manufacturer's instructions.
All results are reported as means±S.E.M. Statistical significance for ELISA, Western blotting and dot blotting data was determined using a two-tailed Student's t test.
Densitometric quantitation of blotting strips was determined using the Quality-One software.
TRAAK protein expression in myometrial samples was determined using Western blot (Fig 2A).
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