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At each probing, the blot was analysed using a PhosphorImager.
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The blots were analysed using a PhosphorImager (GE Healthcare).
Western blots were analysed using image densitometry with TotalLab TL100 software (Nonlinear Dynamics, Durhan, USA).
Blots were analysed using Image J software to semi quantify individual bands.
Fluorescent intensity of bands on western blot and dots on dot blots were analysed using ImageQuant software.
Western blots were analysed using HRP-conjugated secondary antisera (Sigma), or using the Odyssey infrared system (Licor, Lincoln, NE, USA).
The developed blots were analysed using the Image J program to quantitate the intensity of the bands and the values for Mpl expression are shown in Fig. 11.
After washing in 1×SSC/0.1% SDS for 20 min at room temperature and three times with 0.2×SSC/0.1% SDS for 10 min at 65°C, blots were analysed using a phosphor imaging system (Fujix Bas 2000; Fuji Photo Film Co. Ltd., Tokyo, Japan).
After washing in 1 × SSC/0.1% SDS for 20 min at room temperature and three 10-min washes with 0.2 × SSC/0.1% SDS at 65°C, blots were analysed using a phosphor imaging system (Fujix Bas 2000).
The developed blots were analysed using the Image J program to quantitate the intensity of the bands and the values for Mpl expression after normalization against β Actin expression are represented in Fig. 4. Statistical analysis of the values by t-test revealed the reduced Mpl expression to be significant (P < 0.05).
The blots were imaged and the density was analysed using FluorChem®SP imaging software (Cell Biosciences).
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