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Separate blot was also probed for β-actin as loading controls.
To check that Lck was blocked by the inhibitor, the blot was also probed with anti-phospho-Lck antibody.
For the expression studies, the blot was also probed with a primary anti-GFP rabbit polyclonal serum (1 1000; Invitrogen) followed by a polyclonal goat anti-rabbit IgG-HRP secondary antibody (1 2000; Dako North America, Inc., Carpinteria, CA, USA) as a loading control.
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Blots were also probed with U6 probes to normalize loading.
Blots were also probed for actinin (anti-actinin, Sigma).
The biotinylation blots were also probed simultaneously with GluR1 and GluR2 antibodies using the ECL plex system, and subsequently reprobed for actin.
Duplicate blots were also probed with an anti-6His antibody.
Blots were also probed for parkin to ensure pulldown.
Blots were also probed with anti- β-actin (Santa Cruz, CA, USA) to control for total protein content.
The blots were also probed with β-actin antibody for comparison.
Blots were also probed for parkin (sc-30130; Santa Cruz Biotechnology) to ensure pulldown.
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