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Seven categories (1-87) for the Western blot validation have been proposed in which three (grade 1-3) are supportive, two (grade 4-5) are uncertain, and two (grade 6-7) are non-supportive.
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Therefore, the study validation has been achieved.
Validation has been conducted using immunoassay kits.
No external validation has been performed.
Western blot analyses have been published in which potential mutant protein bands were detected for the CREBBP, EP300 [22] and MBD4 genes [23], but there was no validation of these bands.
Validation studies have been undertaken recently [ 4- 6].
Validation studies have been reported previously.
The validation reports have been provided.
All Western blots are cropped from the full length blots that have been included in the Supplemental Material.
For instance, direct sequencing and Northern blotting validations for endogenous small RNAs have been successful in identifying the majority of known miRNAs [26], [30], [31], [32], [33], [34], [35], [36], [37], [38].
Northern blotting and PCR based amplification of adaptor-ligated cDNA have been used for validation of predicted miRNAs [ 10].
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