Sentence examples for blot using a restriction from inspiring English sources

We analyzed the inter-population helena copy number polymorphism by Southern blot, using a restriction enzyme that does not cut inside the element.

To determine the number of gene-trap tTA transposons in each transgenic line, we performed Southern blot analysis using a restriction digest scheme that resolves the concatemerized transposons to a single band and estimated the number of copies based on its intensity (see Materials and Methods).

10 20 µg genomic DNA from the tails of mice was digested with the indicated restriction enzymes and analyzed by Southern blot using a-P32-dCTP labeled random-primed DNA probes (Stratagene Prime-It II kit).

We digested previously isolated lambda genomic clones of the human FGF8 region [ 22] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21] probe, as previously described [ 20].

For lcp3, cutting with the restriction enzymes NotI and AscI (each has a single restriction site in lcp3), was confirmed by Southern blotting using an lcp3-specific probe.

When cleaning your mattress, make sure to blot the stain using a damp white cloth and cold water.

Sixty-five pups were born, two of which were positive by Southern blot using PstI restriction (Fig. S1B) and PCR analyses.

We generated the TCRβDMF transgenic mice and then analyzed the genomic DNA isolated from thymocytes by Southern blotting using BglII restriction enzyme and a probe that spans the Vβ14 gene segment (Figure 6).

Telomere length has traditionally been estimated by Southern blotting using enzymes with restriction sites in subtelomeric DNA.

Intactness of the transgenes was carefully mapped by Southern blot analysis using a total of 11 different restriction enzymes and hybridization with cosmid LCRε, -γγδβ probes [16], and smaller probes along the β-globin locus [11].

To validate the observed restriction digestion DNA hypomethylation results, we performed dot-blot analysis using a 5-methylcytosine antibody.