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Then, the membranes were analyzed by Western blot using a pool of sera from MAP-infected animals.
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Pulsed field gel electrophoresis was used to separate plasmid and chromosomal DNA in B. thuringiensis AWO6, and a Southern hybridization blot using a mixed pool of pXO2-derived prORFs (ORFs 6, 10, 50, 63, 72, 81) was performed to determine if any of the ORFs were present on the pAW63 plasmid.
Blot using a tissue to mop up extra color.
In western blot analysis, CD4 was detected using rabbit anti-CD4 polyclonal antibody H370 (Santa Cruz), while GFP-PLSCR1, -PLSCR3 and -PLSCR4 fusions were detected using a pool of two mouse anti-GFP monoclonal antibodies (7.1 and 13.1, Roche).
After purification, the preparations were analyzed by Western blotting using a monoclonal antibody directed against the E viral protein (Fig. 1d, SD pool as an example).
We revealed the blots using a Supersignal system (Pierce).
Conditioned medium from pools of MSU-1.1 clones obtained after transfection with NPro-cbEGF18-26 NPro-cbEGF18-26 NPro-cbEGF18-26564Y constructs were analysed by western blotting using an antibody raised against the proline-rich region of fibrillin-1 (Fig. 4B).
When cleaning your mattress, make sure to blot the stain using a damp white cloth and cold water.
This scheme uses a pool of keys.
We use a pool of keys and then randomly select some keys from that pool.
The SiarhEPOELD-containing eluates from the 2F5 immunoaffinity chromatography were identified by dot blot using anti-hEPO antibodies and pooled.
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