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Both CDT intoxication and irradiation induced activation of p38 MAPK within 3h after treatment and this effect was maintained for at least 24h, as assessed by western blot using a p38 MAPK phospho-specific antibody (p-p38) (Figure 5A).
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Northern blot using a Lim3A-specific probe revealed two transcripts (Figure 2A).
The presence of CAG repeat sequences with the correct length in the transgenic mice was also checked by PCR-based Southern blot using a (CAG 10 oligomer as a probe (Fig. 1B).
Dephosphorylation was confirmed by western blot using a pSer724 phospho-specific antibody.
Genomic DNA was digested with BamH1 and analysed by Southern blot using a 0.75% agarose gel and the subtelomeric probe pTelBam8 (26, 26).
Saccharomyces cerevisiae PJ69-4A strain cultures were independently transformed with the two bait plasmids and single clones were assayed for protein expression by Western blot using a HESX1 antibody [19].
Successful transfection of SATB2-GFP was confirmed by western blot using a 1 2000 dilution of affinity purified Satb2 polyclonal antibody and a 1 1000 dilution of monoclonal anti-GFP antibody.
Twenty-three isofates of P. destructans from central Europe were screened via Southern blot using a 900-bp PCR fragment of MAT1-1-1 as a radio-labeled P probe according to standard procedures (Sambrook and Russell 2001).
Purity and identity of the proteins were determined by Coomassie staining and Western blot using a 1 1000 dilution of polyclonal mouse anti-His C-terminal antibody (Abcam) following SDS-polyacrylamide gel electrophoresis on an AnyKD (BioRad) gel.
10 20 µg genomic DNA from the tails of mice was digested with the indicated restriction enzymes and analyzed by Southern blot using a-P32-dCTP labeled random-primed DNA probes (Stratagene Prime-It II kit).
ACE2 protein was determined by Western blot using an ACE2 goat polyclonal antibody (R&D Systems).
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