Sentence examples for blot using a mouse from inspiring English sources
Equal loading of protein was determined by Western blot using a mouse anti-β-actin antibody (Novus Biologicals).
Active Rac1 (GTP bound) in the supernatants was detected by western blot using a mouse anti-Rac1 mAb (Cell Biolabs).
The product demonstrated as a 17- and a 34-kDa band; both bands were identified by western blot using a mouse anti-hIL-25 monoclonal antibody.
Caspase-mediated cleavage of PARP was assessed by western blot using a mouse monoclonal anti-human PARP (BD Biosciences) at 1 : 250 dilution.
Then, RAS protein was assessed by western blot using a mouse anti-RAS clone10 antibody (1 : 1000) in GST pull-down and whole lysates (Millipore-Upstate, Billerica, MA, USA) and a goat anti-GST antibody (1 : 1000) or a mouse α-tubulin antibody (1 : 5000) (GE Healthcare), respectively.
The expression of the HA proteins was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions and western blotting using a mouse monoclonal anti-V5 antibody (Invitrogen), or a rabbit polyclonal anti-H5N1 HA1 specific sera.
6X-His tagged CspA, RhlB, SrmB, and CsdA were detected by Western blotting using a mouse monoclonal anti-6X His tag antibody (Abcam, United Kingdom) followed by a goat anti-mouse polyclonal antibody conjugated to HRP (Abcam).
To determine if human NPM3 is similarly located and to identify genomic clones of NPM3, we analyzed FGF8-containing human genomic lambda clones [ 22] by Southern blotting using a mouse Npm3 cDNA probe.
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21] probe, as previously described [ 20].
Expression of WRN or WRN mutant proteins was determined by Western blot using a WRN mouse monoclonal antibody directed against an epitope in a purified C-terminal fragment of WRN [ 47] (1:1000, Spring Valley Labs).
TAP protein was determined by Western blot using a goat anti-mouse TAP1 polyclonal Ab (for TAP1, Santa Cruz Biotechnology, Santa Cruz, CA) or mouse antiserum (for TAP2, generated in our laboratory [36]).
