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The presence of differentially tagged proteins in the IP fractions was determined by Western blot using a mixture of anti-HA and anti-Flag polyclonal antibodies.
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The resulting protein samples were separated on a 96-well gel electrophoresis system, transferred onto a nitrocellulose membrane, and Sup35p and Pgk1p in the samples were detected by western blotting using a mixture of the two respective primary antibodies (Pgk1p levels were used as an internal loading control).
The reaction mixture was incubated at room temperature for 20 minutes, washed three times, then protein samples were analyzed by western blot using an anti-GST antibody (Cell Signaling).
A recent study of Western blotting found that using a mixture of B. burgdorferi sensu stricto and B. afzelii antigens the diagnosis was improved in up to 70% of sera with borderline results from the enzyme-immunoassays using a purified flagella antigen (Zeus Scientific, Raritan, United States) [ 13].
When using a mixture reference panel, we filtered SNPs using a mixture MAF.
After about 2 min adsorption, the sample on the grid was blotted using a filter paper.
The reaction mixture was then subjected to nickel-column purification followed by Western blotting using an anti-SIP1 antibody.
All samples were detected by Western blotting using an anti-His monoclonal antibody.
When cleaning your mattress, make sure to blot the stain using a damp white cloth and cold water.
Then neutralize the stain by pouring a small amount of club soda on a cloth and blotting it on the stain.,, Alternately, you can use a mixture paste of baking soda and water.
Here, I use a mixture of cherries and peaches.
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