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Targeting events were identified by insertion-specific PCR and confirmed by Southern blot using a flanking probe.
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Deletion of the genomic sequence was confirmed by Southern blotting using a flanking probe as well as a neomycin probe (Figure 4B).
Approximately 300 recombinant clones were screened by Southern blotting using a PCR generated 3′ flanking probe in order to confirm the site-specific integration of the LoxP sites (Fig. 1A).
Currently, the number of D4Z4 repeats is usually determined by Southern blotting using a probe that hybridizes to the centromeric flanking sequence, p13E-111.
After washing, the bound proteins were analyzed by western blotting using an anti-Rac1 antibody.
When cleaning your mattress, make sure to blot the stain using a damp white cloth and cold water.
Correct targeting was confirmed in 5.2% of all embryonic stem cell clones by Southern blotting using flanking 5' and 3' probes and a NeoR internal probe.
Southern blots using flanking probes from outside the targeted regions confirmed the presence of the insert (Fig 1D).
Protein quantification was achieved by a quantitative Western blot using anti-Histidine (Invitrogen) antibody.
This blot uses both a PTPRS (rabbit green) and Flag (mouse red) antibody.
For Western blot, samples were loaded onto a NuPAGE® Novex® 4 12% Bis-Tris Gel (Life Technologies) and blotted using in an iBlot Gel Transfer Device (Life Technologies).
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