Blot using a tissue to mop up extra color.
Of 199 carcinoma cases, 73 tumours as well as 44 normal samples were also analysed using a combination of RT-PCR and Southern blot hybridization, Western blot, and mutation gene assays.
In this study, a systematic approach was used to increase glycoprotein production titres of an exemplar protein, AcrA, and the resulting glycosylation efficiency was quantified using a combination of Western blots and pseudo Selective Reaction Monitoring (pSRM).
Specificity was calculated using a combination of Western blots and NAT as the gold standard.
The expression of oto mRNA during development was examined from E5.5-18.5 using a combination of northern blots and in situ hybridization on whole mount and sectioned embryos.
Using a combination of Western blotting and immunohistochemistry (IHC) experiments, p-AKT levels were found to be reduced following PI3K inhibition (Fig 7B and C), while SPRY2 expression was not altered (Supporting Information Fig S21).
This study describes for the first time the proteomic characterisation of cartilage from Matn3 V194D, Comp T585M and Comp DelD469 mutant mice using a combination of Western blotting and semi-quantitative spectral counting (mass spectrometry) and identifies both common and unique disease signatures that also provide insight into detrimental changes that may predispose to cartilage degeneration.
Molecular diagnosis of DM1, using a combination of long PCR and Southern blot analysis in the peripheral blood, showed that DM1 patients considered in this study had a CTG expansion between 800 and 1500 repetitions.
By using a combination of 2D gels, western blots and proteomics, we identified a set of proteins that were recognized by sera from both acute and convalescent patients suffering from meningococcal septicaemia.
By using a combination of qRT-PCR, Western blotting, immunohistochemistry and in situ hybridization, we show that C-RAF and B-RAF are expressed during the early development of the chicken inner ear in specific spatiotemporal patterns.
