Sentence examples for blot using a blaOXA-72 from inspiring English sources

Southern blot using a blaOXA-72 probe further revealed the presence of blaOXA-72 in the plasmids obtained from 27 isolates.

Southern hybridization studies using a blaOXA-181 probe localised the gene to a large plasmid of ~170kb in six of the seven clinical isolates and to a ~194kb plasmid in isolate KPO26 (Table 1).

To examine the copy number of the blaOXA-23 gene in different A. baumannii genomes, we performed Southern blot hybridization on EcoRI-digested DNA fragments using a 589-bp DNA probe specific for the blaOXA-23 gene.

Western blots using R21 revealed a number of immunoreactive bands.

Plasmid was extracted and digested by restriction enzymes, and subsequently analyzed by electrophoresis and Southern blot for blaOXA-72.

In 2 isolates, blaOXA-51 and blaOXA-58-like genes were also simultaneously present, while 2 more carried a blaOXA-51-like gene (Table).

All of the strains were positive for blaOXA-51 (intrinsic resistance), 14/27 strains carried blaOXA-23, 2/27 strains carried a blaOXA-24-like gene, and 4/27 strains had a blaOXA-58 gene.

All of the strains had a blaOXA-51-like gene, and four strains had a blaOXA-58 gene.

Sixty-six imipenem resistant isolates (79.5%) carried IS Aba1 genetic element preceding the naturally occurring carbapenemase blaOXA-66, a blaOXA-51 like gene [GenBank: EU977573, EU977569] (See Additional file 1).

The 10 isolates that belonged to European clone II had a blaOXA-23 gene that was part of Tn 2006.

The molecular analysis of the isolates tested in this study revealed that 14 strains (51.8 %) carried the blaOXA-23-like gene and that two strains carried a blaOXA-24-like gene.