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In this study, we characterized by Western blot the expression of S100B in human tissue using two different antibodies and corroborated this data with mass spectrometry.
Therefore, we assessed by Western blot the expression of E6 and E7 in three human cervical carcinoma cell lines HPV16-positive CasKi and SiHa celines HPV16-positiveositive HeLa S3 CasKiline.
We also explored by western blot the expression of caspase-9 in Bid-overexpressing and parental RA FLS after treatment with anti-Fas or anti-Fas and Wort.
To rule out a possible involvement of Smad 4 and inhibitory Smad 7 in the inhibition of Smad2/3 activity, we analyzed by Western blot the expression of the two Smad proteins in mouse lung lysates (Online Resource 1).
In order to investigate which molecule is present in LAMA84 exosomes that could be responsible for the activation of pro-survival and proliferative pathways, we assessed by Western blot the expression of TGF-β1 in LAMA84-derived exosomes.
We then analysed by Western blot the expression of stathmin, p53 and DNA-PK (Fig 8B) in a panel of 51 Serous Epithelial Carcinomas (48/51 High Grade) consecutively collected in our Institute between 2010 and 2012 (case material C, in Supporting Information Table S3).
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To determine the effect of shRNAs in the expression of a de novo viral protein we evaluated by western-blot the expression of Vif after 48h of HIV-1NL4-3 infection.
Moreover we quantified by western blotting the expression of the enzyme COX-1, since it has been reported that it increases consistently over the full course of MEG-01 differentiation [33].
Using Western blotting, the expression of several proteins potentially involved in RIPC-mediated organ protection was investigated [ 21, 24, 39].
Using western blotting, the expression of EZH2 in A549 and HTB-56 cells was evaluated after siRNA transfection.
As observed by Western blotting, the expression of P2X5 was higher in high grade than in low grade carcinoma.
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