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Since FAK activity is also regulated by phosphorylation at Tyr 925 [19], we analyzed by Western blot the effect of androgens on this phosphorylation.
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By western blotting, the effect of shERα on ERα protein expression was studied (Fig. 1D).
Given the pro-apoptotic effect of 2.7 mM d, l-Hcy, we analyzed by Western blotting the effect of Hcy on NOX2 expression.
We evaluated by western blot analysis the effect of treatment on the expression of proteins involved in the PI3K pathway.
By using quantitative Western blot analysis, the effect of age on endogenous IGF2 levels was measured in the hippocampus of 15-month-old versus 7-month-old wild-type (WT) animals.
(i) Western blotting shows the effect of PI3K inhibition (500 nM GDC-0941) and PDK1 inhibition (500 nM BX-795) on PARD6B and PARD6G loss-dependent phosphorylation of Akt at T308.
Differentially expressed genes were analysed using RT PCR and western blotting, and the effect of anti-cadherin neutralising antibodies on their expression was assayed.
We investigated by Western blotting the effects of bisphosphonates and/or growth factors on phosphorylation states of these kinases in MCF-7 breast cancer cells.
These findings suggest that dFdC may still have inhibition impact on RR. Figure 5: Western blot analysis of the effect of dFdC on expression of ribonucleotide reductase subunits M1 (RRM1) and M2 (RRM2), P53-controlled ribonucleotide reductase (P53R2).
(b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin.
Lysates were prepared for ERK-1 expression by Western blot analysis and the effect of ERK-1 silencing on HCV was assessed by measuring the luciferase gene activity.
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