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Paraffin-embedded sections, immunohistochemistry, immunohistochemical double staining and Western blot techniques were used.
Semiquantitative RT-PCR and Western blot techniques were used to estimate Gai and Gβ mRNA and protein levels, respectively and normalized to an internal standard (β-actin).
Software-guided analysis, RNA cross-link immunoprecipitation (CLIP), 3' UTR luciferase reporter assays, qRT-PCR, focused super-arrays and western blot techniques were used to identify and confirm targets of miR-31.
Standard Western blot techniques were used.
Slot blot techniques were used to study the MUC gene expression and quantitation.
RT-PCR and western blot techniques were used to validate target gene expression.
Similar(53)
Initially, Northern blotting techniques were used for miRNA discovery and detection.
Western blotting techniques were used to analyze equal amounts of protein as described previously [67].
Standard blotting techniques were used [28] except that the concentration of Denhardt's was increased to 10×, SDS to 2% and 200 µg/mL sheared and denatured calf thymus DNA was used instead of salmon DNA.
Standard Southern blotting techniques were used to analyze BamHI-digested genomic DNA from founders and subsequent generations using the 842-bp NcoI -SphI fragment of the tTA open reading frame as a probe.
The dot blot technique was used to determine the sensitivity and specificity of the capture probe.
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