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The isolations of DNA from tail biopsies and mRNA from tissue as well as PCR, Southern, Northern and dot blot techniques were performed as described in Hartenstein et al. (1996).
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qPCR, zymography and western blotting techniques were performed as described in our previously published studies [ 12- 14, 20].
Protein extraction and western blotting technique were performed as described previously [ 18].
Western blot, immunohistochemistry and real-time PCR techniques were performed to measure the levels of GABAA receptor γ2 and α1 subunit transcripts and protein in the nRt and ventrobasal (VB) relay nuclei of thalamus and in somatosensory cortex.
Standard Western blot techniques were used.
SDS PAGE and western blot analyses were performed using standard techniques.
Western blot experiments were performed in independent runs at different laboratories, with each laboratory using its established techniques and procedures.
Western blot analyses were performed utilizing standard procedures.
Total RNA extraction and northern blot analysis were performed.
Western blot analyses were performed according to standard procedures.
Western blot analyses were performed as previously described33.
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