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Additionally, the RecA protein was measured by the Western blot technique using a specific antibody.
To further substantiate the results, all sera were analysed by the Western blot technique using a cell lysate of PancTu-1 cells (p53 mutation at codon 176) as antigen source.
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Analysis of Ig gene rearrangements by the Southern blotting technique using a JH probe established that the variants and the original tumor cells were all clonally related.
Additionally, all positive sera were analysed by the Western blot technique using recombinant p53 as the antigen source.
Confirmation of the single integration event of the pC-P54::MeCu/ZnSOD-35S::MeAPX2 T-DNA in these transgenic lines were carried out by the Southern blotting technique using XbaI-digested cassava genomic DNA, which were extracted from leaves of in vitro plants and hybridized with DIG-labeled HPT probe.
The fact that we detected reductions in GBA messenger RNA levels in cells bearing the L444P mutation probably reflects the greater sensitivity of the quantitative PCR technique we used, compared with the northern blot techniques used in earlier studies.
In regions from developing nations with a estimated TB prevalence of 5%10%%, described in out-patient units attending persons with coughs for more than three weeks (respiratory symptomatic, according to WHO), NPV for the PCR colorimetric dot-blot technique used in parallel with a high clinical suspicion of SNPTB, ranged from 98 to 99%, among HIV seropositive and HIV seronegative subjects.
Hsp20 expression was analysed using standard SDS-PAGE and Western Blotting techniques using the phospho-Hsp20 antibody (ab58522 Abcam, UK) and alpha-tubulin-HRP antibody (ab40742 Abcam, UK) as the loading control.
A Western blotting technique was validated using a GST XIAP fusion protein as a standard and HeLa cells and SF268 (human glioblastoma) cells as high and low XIAP expression QCs.
Further, a western blot technique was used to partially quantify the expression of P-selectin and CD45 on the surfaces.
The dot blot technique was used to determine the sensitivity and specificity of the capture probe.
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