Sentence examples for blot screening of a from inspiring English sources

Using antibodies against rat liver cEH, 24 positive clones were picked upon colony blot screening of a pEX 1/E. coli POP 2136 expression library.

In a Western blot screening of DFF45/DFF35 content in P31 and P31res1.2 cells, no changes were identified under control conditions (data not shown).

Five clones were selected for immunization and all yielded functional antibodies that gave specific staining in Western blot screening of samples from various cell types.

Thirty-five potential FT orthologue-containing BAC clones were identified by Southern blot screening of the JBnB BAC library, which was developed from the doubled haploid B. napus cv.

The use of dot blot screening of supernatants allows the rapid identification of vectors or conditions yielding sufficient secreted protein to proceed successfully through purification when grown in a larger scale.

The 3′ Southern blot screening is based on digestion of genomic DNA with NheI and hybridization of an external 523 bp probe downstream of the 3′ homology sequence of the mFwe targeting vector.

Figure 6 shows the screening of a subset of mutants by western blot.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) coupled with Western blot analysis confirms the results of the initial dot-blot screen, where four-of-four fast growers show a protein band consistent with Cel7A as compared with an immune-reactive band from wild-type QM6a grown in lactose-containing medium for cellulase induction.

Southern blot screens were used to characterize complexity of all lines (data not shown).

Whole genome sequencing or screening and mapping of genomic libraries has confirmed single LWS opsins in two species of pufferfish [ 29], and two LWS opsins in both the zebrafish [ 16] and medaka [ 17], while screening and Southern blotting of a genomic library suggests the presence of two (or possibly three) LWS loci in the cavefish (Astyanax fasciatus) [ 21].

Using a western blotting screen, we observed that the phosphorylation of MKK4 and JNK induced by I/R was also lower in the absence of TRAF1, although the total protein levels of these kinases were not altered in TRAF1-KO and WT groups.