Sentence examples for blot protocol from inspiring English sources

In rat brain tissue, however, SCN8A protein (NaV 1.6) was easily detectable using an identical Western blot protocol (Figure 5). Figure 5 Analysis of SCN8A protein expression.

The standard Southern blot protocol was utilized.

Western blot protocol was described previously [36].

Further details on the western blot protocol can be found in Supporting Information S1.

Thereafter, the membranes were further processed according to the paraffin-embedded tissue (PET) blot protocol described elsewhere [32], [33].

Details of the Western blot protocol used in the present study can be found in a previous publication [13].

While a Northern blot is an effective tool, traditional Northern blot protocols are complicated, time-consuming, and usually inconvenient for users.

Hybridizations with a radioactively labeled kDNA probe were performed as recommended by standard Southern blot protocols [65].

Analysis of sur-5::gfp mRNA levels was performed using standard northern blot protocols (1.2% agarose/formaldehyde) on poly(A+) RNA.

Equal quantities of protein were subjected to NuPAGETM 4 12% Bis-Tris Gel (Invitrogen, Carlsbad, CA) and were analyzed with standard Western blot protocols (HRP-conjugated secondary antibodies from Santa Cruz Biotechnology and ECL from Amersham Biosciences, Buckinghamshire, UK).

Plasmid constructs, cell labelling and western blot protocols were as described previously (supplementary information online).