Sentence examples for blot procedures were used from inspiring English sources

The same SDS-PAGE and western blot procedures were used to directly detect proteins in 15 µg of total extract prepared in NET-Tx100 lysis buffer or in 1 µg of raft proteins prepared in 6% RIPA buffer.

Routine Western blotting procedures were used to detect protein content as described10.

Standard western blotting procedures were used as previously described [ 8].

Routine Western blotting procedures were used to detect protein content as described previously [ 30].

Proteins (30 μg/sample) were separated via reducing 10% SDS-PAGE and standard western blotting procedures were used to detect proteins of interest with the following primary antibodies: PDK2 (Cat# AP7039b, Abgent, San Diego, CA, USA), β-actin (Abcam), Noxa (OP180, Calbiochem) and Puma (ab9643, Abcam).

For Western blotting, standard immunoblotting procedures were used [68].

Similar procedures were used for Western blot expression assessment of TGF-β1 (anti-TGF-β1, Santa Cruz, Biotechnologies, Santa Cruz, USA), phospho-Smad (anti-Smad2, phospho-specific, Calbiochem, Schwabach, Germany) and phospho-ERK (anti-active ERKMAPK, Calbiochem, Schwabach, Germany).

The probe was synthesized (using cDNA derived from SH-SY5Y total RNA as a template) and Northern blot procedures were performed using DIG Northern Starter Kit according to manufacturer's instructions.

The western blot procedures were performed using ECL or ECL plus (Amersham).

Standard western blotting procedures were followed using Tris-glycine SDS-PAGE and electrotransfer onto PVDF membrane at 100 V for 1 h at 4 ºC.

A standard Southern blot procedure was followed using CDP Star detection (Roche) with AP-conjugated anti-Dig Fab fragments (Roche).