Sentence examples for blot over from inspiring English sources

Complementation crosses with ap blot over a set of overlapping ap deletions mapped the mutation to am ∼11-kb interval upstream of ap MM.

A further significant advantage of Western blot over MS is the fact that the same antibodies can be utilized for confocal microscopy analysis, adding important information on the localization and expression pattern of the analytes in a certain tissue.

Mug20-GFP was detected by incubating the blot over night at 4°C with goat anti-GFP antibody (1 2,000, Rockland, Gilbertsville, PA) in TBST (20 mM Tris pH 7.5, 140 mM NaCl, 0.05% Tween 20) + 3% dry milk.

Membrane were washed with PBS supplemented with 0.1%Tween200 (PBS/T), blocked in buffer containing PBS/T and 5% fat-free powdered milk (Marvel) or 5% BSA and blotted over-night with primary antibodies in blocking buffer.

Indeed the expression level of osa-miR159a.1 osa-miR159a.1orthern blot is over-evaluatedetected anti-sense miR159a.1 proby probably cross-hybridises with other members of this family.

He held her chin and spread the muck back onto her face, blotting over the startling line of her brows, the perfect symmetry of her eyes and nose, the devastating curve of her lips.

Culling pictures from gay pornography sites, he electronically blotted over the naked bodies to remove all taint of sexuality and called viewers' attention to the nasty bits: the clashing light switch plate, the stereo speaker used as a shelf, the pile of magazines splayed on the floor.

Treatment with the proteasome inhibitor also significantly increased expression of total β-catenin protein by western blotting over a range of concentrations.

Although we also attempted to knock down TIP60 transcripts using siRNA in garcinol-treated cells, we did not observe a reduction in TIP60 protein levels by western blotting over the time course of the experiment (data not shown), thus we were unable to establish definitively whether TIP60 is responsible for the observed increase in H4K16Ac.

The DNA samples were depurinated in 0.25 mol l−1 of HCl, denatured in 0.4 mol l−1 of NaOH/3 mol l−1 of NaCl, and transferred to a positively charged nylon membrane Hybond-N (Amersham Pharmacia Biotech, Little Chalfont, UK) by capillary blotting over 12 h.

We have observed slight activation of Chk1 when western blots are over-exposed, but this level of phosphorylation is far lower than observed after replication forks have collapsed as a consequence of Chk1 inhibition.