Thus, taking into account that the only available data were related to the amount of monomers, dimers and the sum of all oligomers of ASYN (restricted by the western blot method used to detect them), part of the system could not be directly parameterized.
When using the Immunetics ELISA as a screening tool, it becomes clear that, irrespective of the blot method used, samples with an index >4 are almost always blot-positive.
Patients' and controls' HLA Class I (A, B) and Class II (DRB1) genotypes were determined by the reverse dot blot PCR-SSO method, using the commercially available RELI™ SSO kit (DYNAL BIOTECH A.S.A, Oslo, Norway).
Patterns of recognition of brain proteins by plasma IgG were detected by quantitative immunoblotting (PANAMA-blot method), using a protein extract from the brain of a healthy individual as the source of antigens as previously described [26], [27].
The semi-quantitative western blotting method using synthetic mouse sarcolipin was validated by estimating the levels of sarcolipin in the atria of FVBN mice.
After protein quantitation using the Lowery protein assay, equal amounts of proteins were separated by SDS-PAGE and blotted onto nitrocellulose membranes by the semi-dry blotting method using a three-buffer system.
The methylation states were analyzed by Southern blotting method using a methylation-sensitive enzyme, HpaII, and a partially methylation-sensitive isoschizomer, MspI.
Western blot methods used to identify and characterize PrPres in TgOvPrP4 mice have been previously described (19, 20 ).
For specific HEV antibody detection in swine, we developed ELISA and Western blotting methods, using a truncated capsid (ORF2) protein lacking the first 111 amino acids of a swine HEV genotype 3 strain.
Western blot was performed with standard methods, using the enhanced chemiluminescence developed previously by Haan and Behrmann [ 114].
