N7-methylguanine in DNA was quantified by an immunoslot blot method and using the antibodies as previously described (Harrison et al, 2001) with one modification: instead of whole tissue/cells, DNA was isolated from sonicates.
After four times washes with lysis buffer, the precipitated materials were analyzed by western blot method using the indicated Abs.
For the detection of these protein aggregates, the most sensitive PET blot method was used according to the protocol by Kramer and Schulz-Schaeffer [ 23].
Western blotting was performed with conventional methods by using 25 μg total or cytoplasmic protein or 15 μg nuclear protein.
Thus, taking into account that the only available data were related to the amount of monomers, dimers and the sum of all oligomers of ASYN (restricted by the western blot method used to detect them), part of the system could not be directly parameterized.
The sensitivity of this ELISA test for αSD detection was compared to the previously described Western-blot method [ 5], by using both tests to examine serial dilutions from the same sick M83 mouse brain.
To determine whether the cloned Rf4 reduced rpl5-orf352 transcripts, we performed northern blot analysis by using RNA extracted from the leaf blades (Additional file 1: "Methods").
An alternative to using whole cell lysates for WB detection of anti-DFS antibodies, is to confirm the DFS-IIF pattern by dot blot or line blot methods that use the full length DFS70/LEDGFp75 or a truncated C-terminal fragment of this protein containing the autoepitope region [22].
The methylation states were analyzed by Southern blotting method using a methylation-sensitive enzyme, HpaII, and a partially methylation-sensitive isoschizomer, MspI.
Patterns of recognition of brain proteins by plasma IgG were detected by quantitative immunoblotting (PANAMA-blot method), using a protein extract from the brain of a healthy individual as the source of antigens as previously described [26], [27].
