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"We call it a dot blot kit," Solomon says.
The Western Blot Kit, the Bax and Fas proteins, and supplementary reagents were purchased from Proteintech (Chicago, IL, USA).
The western blots were revealed by chemiluminescence (RENAISSANCE, Western blot kit, NEN Life Science (Boston, MA)).
The reactive bands were visualized using an ECL Plus™ Western blot kit (PIERCE, Rockford, IL) [55].
Specimens with repeatedly reactive EIA results had been tested using the Genetic Systems HIV-1 Western blot kit (Bio-Rad, Redmond, Washington).
Immunoblotting was performed using the WesternDot 625 goat anti-mouse Western blot kit (Invitrogen) according to the manufacturer's instructions (Text S1).
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MTT and western blot kits were from Sigma.
Membranes were processed with WesternDot™ 625 Western Blot Kits (Life Technologies) as described by the manufacturer.
The signals were detected by BM Chemiluminescence Western Blotting kit (Roche, Mannheim, Germany).
All blots were developed using ECL Western blotting kit (GE Healthcare, Munich, Germany).
The signal was developed with the SuperSignal Western Blotting kit (Pierce) on X-ray film (GE Healthcare).
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