HO-1 induction was verified by immunocytochemistry and western blotting in response to hemin (similar results obtained for CoPPIX; data not shown).
(B ) Inhibitor dose response Western blots in response to PDGF signaling show activation of c-Jun N-terminal kinase (JNK) in response to MEK/ERK inhibition but (B' ) no activation of ERK following PI3K inhibition.
Neurons were cultured for 8 or 48 h, and O-GlcNAc modifications and PKA substrate phosphorylation were examined by Western blotting in response to treatment with forskolin, 9d, or a combination of these [ Fig. 6(A)].
Surgically removed H1975 and H1993 tumors were homogenized and tested for levels of key glycolytic proteins (a) and subunits of mitochondrial complexes (b) by western blot analysis in response to each treatment.
However, most of the miRNAs, such as sma-miR159a, sma-miR171b and sma-miR169a, were upregulated in the Northern blot analyses in response to the NaCl treatment (Fig. 3), which is similar to the results of the abundance analysis (Fig. 2) except for that of the miRNA sma-miR396b, which did not present changes in its levels.
Western blot analysis revealed that, in response to vascular injury, TNF-α stimulation induced p65 and STAT3 phosphorylation and promoted translocation of these molecules into the nucleus.
Typical approaches (e.g. western blot) for investigating signaling in response to stimuli require a priori knowledge of specific targets and the availability of phospho-specific antibodies, which limits the ability to globally assess cellular signaling events.
Because survivin may directly bind and inhibit caspases, we tested by western blotting whether survivin decrease in response to Notch2 activation was implicated in N2ICD-dependent caspase activation.
RT and SH performed the RNA gel blots of DIR1 expression in response to hrpPst.
Plasmids of 117 colonies that were positively screened in blot hybridization, indicating induced expression in response to LPS challenge, were isolated and sequenced.
