Sentence examples for blot hybridization using an from inspiring English sources
Twenty-one dpositivetinfectionsions were confirmed by Western blot hybridization using an anti-coat protein antibody (data not shown).
Purified protein was visualized by SDS-PAGE and Western blot hybridization using an anti-His antibody (Fig. 3).
Samples were digested with the methylation-sensitive restriction enzymes HpaII or MspI, a methylation-insensitive isoschizomer, and analyzed by Southern blot hybridization using an IAP-LTR probe [21] (Figure 3).
Electroporated ES cells were selected in tissue culture medium containing G418 and screened for KRIT1 homologous recombination by EcoRV DNA digestion and Southern blot hybridization using a 700 bp KRIT1 fragment downstream of the targeting vector as probe.
The DNA fragments were transfer, UV-linked to a Zeta-probe GT membrane (BIO-RAD) followed by Southern blot hybridization using a 669 bp labeled probe.
Validated methods included pulsed field gel electrophoresis (PFGE), variable number of tandem repeats (VNTR) typing, and Southern blot hybridization using a set of MA DNA probes, including those for typing the vpma genes repertoire.
DNA extracts from clones containing plasmids with inserts were subjected first to southern blot hybridization using a random hexamer prime DIG-labeled normal shrimp DNA probe to eliminate clones containing shrimp sequences [ 25].
Current studies revealed no basal rates of HBD-2 gene and protein-expression in normal human kidney, salivary gland, small intestine or liver [ 5], except one study by Bals and co-workers reported HBD-2 mRNA expression in kidney extracts by dot blot hybridization using a commercially available filter [ 9].
