Sentence examples for blot hybridization using a from inspiring English sources

Electroporated ES cells were selected in tissue culture medium containing G418 and screened for KRIT1 homologous recombination by EcoRV DNA digestion and Southern blot hybridization using a 700 bp KRIT1 fragment downstream of the targeting vector as probe.

The RNA quality was assessed by northern blot hybridization using a Drosophila α1-tubulin probe [ 35].

We confirmed the mutation by Southern blot hybridization using a gene-specific probe and tested the strain for cheating.

The DNA fragments were transfer, UV-linked to a Zeta-probe GT membrane (BIO-RAD) followed by Southern blot hybridization using a 669 bp labeled probe.

Validated methods included pulsed field gel electrophoresis (PFGE), variable number of tandem repeats (VNTR) typing, and Southern blot hybridization using a set of MA DNA probes, including those for typing the vpma genes repertoire.

DNA extracts from clones containing plasmids with inserts were subjected first to southern blot hybridization using a random hexamer prime DIG-labeled normal shrimp DNA probe to eliminate clones containing shrimp sequences [ 25].

Twenty-one dpositivetinfectionsions were confirmed by Western blot hybridization using an anti-coat protein antibody (data not shown).

Purified protein was visualized by SDS-PAGE and Western blot hybridization using an anti-His antibody (Fig. 3).

Samples were digested with the methylation-sensitive restriction enzymes HpaII or MspI, a methylation-insensitive isoschizomer, and analyzed by Southern blot hybridization using an IAP-LTR probe [21] (Figure 3).

Presence of conserved hypersensitive reaction/pathogenicity (hrp) genes (hrcRST) was assessed both by PCR using primers designed to amplify the corresponding sequence and by dot-blot hybridization using a PCR-amplified hrcRST fragment as a probe.

Next, 193 clones were amplified by PCR and analyzed by dot-blot hybridization using an Alu probe.