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The results of these Western blot experiments were validated in IHC experiments that demonstrated decreased levels of caveolin-1 and increased levels of CCR1, CCR2, and CCR3 in AA and SSc monocytes compared to C monocytes (Fig. 2).
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The experiment was validated with AmBisome®.
Western blot experiments were independently performed three times.
Western blot experiments were performed according to the standard procedures.
The western blot experiments were performed in triplicate.
Western blot experiments were performed as previously described [ 20].
Western blot experiments were performed as independent replicates.
Western blot experiments were repeated at least three times.
Western blot experiments were performed by standard methods.
Western blot experiments were performed in six-well plates.
Western blot results were validated by qRT-PCR analysis.
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