Sentence examples for blot experiments were used from inspiring English sources

Averaged data from triplicate adhesion, transwell migration, and Western blot experiments were used to generate bar graphs depicting average values ± standard deviations, with paired Student t tests used to measure significance.

Fluorescence activated cell sorting and Western blotting experiments were used to evaluate the P140 effects on autophagic flux markers.

Western blotting and transfection experiments were used to investigate the mechanism of action of plumericin.

Additionally, western blots, immunoprecipitation, and competition experiments were used to link BAAV's ability to bind chitotriose (a trimer of β-1-4-linked N-acetyl glucosamine) with the post-translationally modified glycoprotein gp96, suggesting that this protein can serve as a receptor for BAAV transcytosis.

All western blot experiments were performed using three independent cultures unless stated otherwise.

Statistical analysis of RT-qPCR expression levels and densitometry levels for Western blot experiments were analyzed using unpaired Student t-tests.

Northern blot experiments were carried out using 5 µg RNA/sample run on 1% agarose gels.

Western immunoblot and nondenaturing dot blot experiments were pursued by using brain, proventriculus, and adrenal homogenates of the 3 animals that were quantitated by real-time PCR, and 2 rabbit polyclonal antibodies raised against recombinant BDV strain V nucleoprotein (N) or P, as well as immune sera from BDV He/80–infected rats.

All immunofluorescence localization and western blot experiments were repeated at least three times using samples from different experiments, and representative images are presented.

The positive and the negative controls for the Northern blot experiments were synthesized by in vitro transcription using the "TranscriptAid T7 High Yield Transcription Kit" (Thermo Scientific, Germany), according to the protocol supplied by the manufacturer.

Dot blot experiments were performed following the same procedure; we used 1 to 3 µg of freshly dissolved monomeric proteins and 3 to 4 µg of fibrillar materials per dot blot.