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All Western blot experiments were conducted in triplicate and results were compared using the Student's t-test, p<0.05.
Five sets of independent quantitative Western blot experiments were conducted with HEK293T cells.
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Cell culture western blots experiments were conducted in two to three independent experiments consisting of duplicates or triplicates.
RNA gel-blot experiments were conducted to determine whether TbDHN1 transcription levels increase in the same conditions affecting plant DHNs transcription, e.g. low temperature stress and salinity stress.
Unfortunately, no satisfactory xCT antibody for western blotting was available at the time these experiments were conducted, thus precluding the examination of xCT protein expression in our studies.
Three independent experiments were conducted and three sets of data were used for statistical analysis of western blot data.
These experiments were conducted with two different siRNA combinations directed against both C/EBPβ and δ, and a representative Western blot is shown in Figure 6F.
Duplicate experiments were conducted.
Two experiments were conducted.
Various experiments were conducted.
Second, more experiments were conducted.
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