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Northern blot experiments were carried out using 5 µg RNA/sample run on 1% agarose gels.
Whole cell lysates of cells or tumor tissues were made and Western blot experiments were carried out as we described previously [26].
Western blot experiments were carried out to study the PBP2a expression in treated MRSA cultures.
Western blot experiments were carried out when the appropriate titer (1 5,000) was obtained following booster doses.
Western Blot Analysis Western blot experiments were carried out as described (Drinjakovic et al., 2010 ) using antibodies against LB2 (1:1000) or α-tubulin (1 3000; Abcam).
Western blot experiments were carried out to measure the intracellular NCAM protein content in drug resistant versus non-resistant tumor cells.
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Three independent Western blotting experiments were carried out for each treatment group.
Densitometric analysis of band intensities obtained from western blotting experiments were carried out using ImageJ software (National Institutes of Health (NIH), USA).
To evaluate the consistency of the assembly and possible recombination across repeats, a Southern blot experiment was carried out.
The Southern blot experiment was carried out with 20 μg of DNA from PCR positive plants digested with the following three restriction enzymes at the same time: PvuII, EcoRI and ClaI.
GST pull-down experiments were carried out followed by Western blot with specific antibodies.
More suggestions(17)
blot experiments were homogenized
blot transfers were carried
blot experiments were validated
blot experiments were run
blot experiments were conducted
blot experiments were amplified
blot experiments were synthesized
blot experiments were designed
blot experiments were performed
blot experiments were done
blot experiments were pursued
blot hybridizations were carried
blot experiments were expressed
blot experiments were reproduced
blot analyses were carried
blot experiments were used
blot experiments were repeated
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