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As an independent test of a change in the splicing pattern, the same RNAs used in the Northern blot experiments were analyzed by qRT-PCR.
Statistical analysis of RT-qPCR expression levels and densitometry levels for Western blot experiments were analyzed using unpaired Student t-tests.
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In CXCR2-signaling analysis of LIM-LIM-CAAX and LASP-KD5 cells, the Western blots from 3 independent experiments were analyzed using NIH Image J program (ver 1.41o).
Results from three independent experiments were analyzed by Western blot, and the intensity of the bands was quantitated by densitometry, using AlphaInnotech FluorChem® HD2 instrument (Alpha Innotech, San Leandro, CA, USA) and analyzed using alphaeasefc software, provided by the manufacturer.
Coimmunoprecipitation experiments were analyzed by western blotting.
RIPA-solubilized mitochondrial fractions, concentrated cytosolic fractions, RIPA-solubilized total cell lysates, inputs, and elutes from immunoprecipitation experiments were analyzed using western blotting (WB).
2002 and 2003 experiments were analyzed separately.
Three sets of independent experiments were analyzed.
Five experiments were analyzed.
Pacific and Atlantic experiments were analyzed separately.
All of the Western blot experiments were repeated three times individually and representative blots are presented in the figure 7 I. Caspase-3 activity was analyzed using CaspACE Assay system, Colorimetric (Promega, Madison, USA) according to the manufacturer's protocol.
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