Sentence examples for blot experiments using the from inspiring English sources

We performed Western blot experiments using the mAb200 RasGAP antibody.

To determine whether SALL4A binds to the Mi-2/NuRD complex as well, and whether endogenous SALL4 in general binds to this complex, we did coimmunoprecipitation and Western blotting experiments using the extracts from SALL4A-expressing 293T cells and mouse embryonic stem D3 cells with high endogenous SALL4 expression.

The previous analysis estimated the copy number with quantitative Southern-blot experiments using the complete DIRS1-like sequence as a probe.

To determine the concentration of PKCαβ and thereby the relative number of rod BCs, we conducted Western blotting experiments using the monoclonal mouse anti-PKCαβ clone MC5 antibody essentially as described previously (He et al. 2000; Osborne et al. 1992).

To test whether the disrupted organisation of the ER and the Golgi apparatus in hau and gho embryos may affect correct modification of proteins running through the secretory pathway, we performed Western blot experiments using antibodies against the membrane-bound Knickkopf (Knk) protein, or the secreted Serp protein (Figure 10).

Northern blot experiments using oligonucleotide probes targeting the 16 novel sRNA candidates mentioned above were performed (Table S3).

Dot blot experiments using an antibody specific to the polyglycerophosphate moiety of LTA confirmed its presence in the mCD36ED-LTA complex (Figure 2D).

Western blot experiments using epitope specific antisera confirm that the corresponding linear amino acid sequences are available for immunorecognition in the engineered protein.

Western blot experiments using a specific anti-human HIF2α antibody confirmed the nuclear expression of the transfected construct in HIF2α clones (Fig. 1c, lower panel).

For this reason, we performed western blot experiments using a specific primary antibody able to bind tau protein on the phospho-Ser396, which is recognized to be the phosphorylation site mainly related to neurofibrillary pathology in AD brain.

In order to verify the deletion of PaMth1 at the protein level, total- and mitochondrial protein samples of juvenile and senescent strains of both the wild-type strain and of the selected transformants were analysed by Western blot experiments using a PaMTH1 specific polyclonal antibody.