Sentence examples for blot experiments not shown from inspiring English sources
Also, the recombinant MVP could be detected in similar Western blot experiments (not shown).
DNA sequences similar to bfpA were detected in 14 (14.1%) of the 99 strains studied, however, only 2 expressed Bfp in Western blot experiments (not shown); these two strains also carried perA and presented AA/LA in 3 hours.
The use of commercial mouse monoclonal antibodies against actin (β-actin C4 from Santa Cruz) was also unsuccessful as these antibodies failed to detect trypanosomal actin either in blotting or immunofluorescence experiments (not shown).
It is worth noting that the bceBloopYtsDBlri strain overexpressing BceR was not found to be resistant to bacitracin (Table 1) even if the chimeric ABC transporter was membrane detected by western blot experiments (data not shown).
Similar levels of γH2AX expression were also observed between pre-adipocytes and adipocytes after exposure to IR (10 and 50 Gy) as measured by Western Blot experiments (data not shown).
This antibody recognises specifically the B and not the A variant in western blot experiments (data not shown).
The resulting P. furiosus mutant with the deletion of PF1950 (MUR27Pf) was verified by PCR and Southern blot experiments (data not shown).
The decrease in Myomegalin-specific staining by siRNA#2 was weak, as expected from Western blot experiments (data not shown; Fig. 1B).
After verification of the resulting mutant MUR23Pf by PCR and Southern blot experiments (data not shown), the mutant strains, MUR23Pf and MUR27Pf, and the wild type of P. furiosus were grown in SME medium in the presence of 0.5% colloidal chitin, 0.025% yeast extract and 0.025% peptone.
However, despite several attempts, EWI-2wint expression in cellular clones could not be detected by cell surface biotinylation and immunoprecipitation or by western blotting experiments (data not shown), suggesting that this protein was poorly expressed in these cells.
In our system the Lhcb1 protein level remained unchanged during the experiment (Western blot data not shown).
