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Western Blot experiments indicate that treatment of cells with cyclopamine decreased the hyperphosphorylated form of pRb (P-pRb) while increasing its hypophosphorylated form (Figure 5A).
Western blot experiments indicate that TMps express larger amounts of VEGF than NMps do (Fig. 3a).
The Southern blot experiments indicate that particle gun transformation of E. elegans often leads to more than one integration event, but the number of integration events is quite low (5 or fewer events).
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Western blot experiments indicated that primary-cultured striatal neurons that were pre-incubated with 25.0 μM LY294002 for 5 min prior to 10.0 μM VU1545 stimulation for 5 min exhibited no Akt phosphorylation above basal levels (data not shown).
The western blotting experiments indicated that all proteins were expressed in M. smegmatis cells, as shown by appearance of correct sized bands for the appropriate proteins (data not shown).
Mutation of Q139, P140 and D141 to glycine residues or eliminating the entire loop (mutant LcΔ) abolished binding of F1-40 thethe Lc peptide in both the competition ELISA and Western blotting experiments, indicating that the QPD triad is necessary for the binding of F1-40 thethe light chain of BoNT/A.
Western blot experiments indicated the expression changes of apoptosis-related proteins, including up-regulation of Bax and caspase-3/9, as well as down-regulation of Bcl-2.
Laboratory experiments indicate that crystalline ice forms only at temperatures warmer than minus-260 degrees.
This in vivo electron microscopy data, combined with Western blotting and transfection experiments, indicates that native PLP co-localizes with mitochondria.
Promoter mapping experiments indicated that each fadD had an independent transcriptional start site, suggesting that they were independently transcribed; however, northern blot analyses indicated that fadD2 and fadD1 can be co-transcribed on a single larger transcript or as smaller independent transcripts (Fig. 3A and 3B).
Western blotting experiments also indicate that NRG1 is downregulated at the protein level.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com