Sentence examples for blot experiment using a from inspiring English sources

To study the expression of the lmo0303 gene, which is expected to be co-transcribed with lmo0302, we performed a Northern blot experiment using a radio-labeled probe directed against lmo0303 RNA.

The relative lack of RNA made it possible only to make one northern blot experiment using a small number of samples.

In order to analyze the composition and amount of OPA1, we performed a western blot experiment using an anti-OPA1 antibody that specifically recognizes five OPA1 species in skeletal muscle, namely isoforms 1 and 7, and short forms 1 3, resulting from proteolytic cleavage.

Further Western blot experiments using a protocol optimized to improve size discrimination across this mass range yielded a band for the cleavage product at ∼30.1 kDa (Figure S9b).

We performed Western blot experiments using a commercial anti-PPARγ antibody.

The reactivity of the VMCH-p8 antiserum was demonstrated in Western blot experiments using a recombinant GST-VMCH-p8 protein produced in bacteria.

Western blot experiments using a specific anti-human HIF2α antibody confirmed the nuclear expression of the transfected construct in HIF2α clones (Fig. 1c, lower panel).

To investigate secretion efficiencies (i.e. the fraction of produced protein that is secreted), comparable amounts of cell and supernatant fractions were analyzed by Western blot experiments using a polyclonal antiserum against NucA.

For this reason, we performed western blot experiments using a specific primary antibody able to bind tau protein on the phospho-Ser396, which is recognized to be the phosphorylation site mainly related to neurofibrillary pathology in AD brain.

In order to verify the deletion of PaMth1 at the protein level, total- and mitochondrial protein samples of juvenile and senescent strains of both the wild-type strain and of the selected transformants were analysed by Western blot experiments using a PaMTH1 specific polyclonal antibody.

In the present work, we furthered this observation, showing that the BCAL2958 protein is expressed by 12 strains belonging to 7 different Burkholderia species isolated from CF patients with different geographical origins, based on western blot experiments using an antibody raised against B. cenocepacia J2315 BCAL2958.