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Blot density of a binary image of Figure 5d was calculated using ImageJ.
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The western blot density of each protein band was quantified using the ImageJ software.
Figures 4A and B demonstrated that relative blot density of pERK/ERK and pAkt/Akt in response to ECa 233 at the concentration of 0.1 μg/ml was not different from that of non-treated group.
The blot densities of the proteins of interest were normalized to those of GAPDH in the same membrane.
The optic density of a 25 kDa-band blotted with anti-NGAL antibody was lower in pigs with advanced HF as compared to controls).
After Western blotting, the density of scanned signals was measured with UN-SCAN-IT gel 6.1 software (Silk Scientific).
Western blot analysis confirmed that the density of the oxidized proteins-positive band (a marker of oxidative stress) increased in a time-dependent manner (P < 0.05, Figures 4(g) and 4(h)).
In the example blot, the integrated optical density of the band decreased by approximately 40% between 1 and 2 wks.
Moreover, steroidogenesis study by Northern blot showed that the density of mRNA of p450scc, p450c17 and 3βHSD at each period was increased with time, but was also inhibited by PD098059 as shown in Figure 4A.
For densitometric analysis of western blot images, density profiles of the bands were measured using ImageJ software.
The first band in each blot was given an arbitrary density of 1, and relative densities of the remaining bands were determined.
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