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Western blots confirmed the findings that no patient had anti-nRNP 70 K antibodies as an initiating autoantibody alone and that in no instance did anti-nRNP 70 K appear before anti-nRNP A by Western blot (data not presented).
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The first control, mock biotinylated CHO cells, were carried through the biotinylation procedure without the addition of the biotinylation reagent and none of antibodies, SERT-, vimenorn- nor pS56-Abs, recognized proteins from these blots (data not presented).
(Data not presented).
Western blotting analysis of samples of diet after N. lugens feeding showed that ButaIT/GNA was stable in the diet over a 2-day interval, with only a small amount of proteolysis of the fusion observed (data not presented).
152 Although dystrophin exon 19 skipping was demonstrated in RNA extracted from lymphocytes after the infusion, the dystrophin staining was equivocal, and Western blot data was not presented.
Vitamin D receptors were present in this patient's tumour as determined by Western blot (data not shown).
We also did not observe any appreciable differences in the amount of calpain present within the cytosolic and nuclear fractions of these cells by Western blot (data not shown).
This was confirmed by Southern blot (data not shown).
All antibodies were tested for specificity by Western blot (data not shown).
Complementarily, the expression of PhoP was tested by western blot (data not shown).
Single integration of the deletion construct was verified by Southern blot (data not shown).
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