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As a reporter of Dad1 promoter function, a cDNA coding for rat CD2 (rCD2), also missing its cytoplasmic tail [27], was recombined into the BAC in frame with the Dad1 ATG start codon, thus replacing most of Dad1 exon 1. Figure 8 shows northern blot data demonstrating that rCD2 mRNA expression from the BAC is widespread and readily detectable in multiple organs.
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Western blot data demonstrated that PDT with 5 mg/L photosensitizer for 3 h at 5 J/cm2 resulted in higher level of active form of caspase-3 (20 kD) in both nanoscale Photosan and conventional Photosan-treated samples (Figure 3A).
Our western blot data demonstrated that 10 μM HNE heightened Egr-1 protein expression in a time-dependent manner.
Indeed, the western blot data demonstrate that the PKA-specific target sites at PLN (S16) and RyR2 (S2808) are hyperphosphorylated in ATX-II-treated atrial myocardium.
Our in vivo studies were consistent with the in vitro data: the western blotting data demonstrating that the major products were the uncleaved [GFP-F2A20-CherryFP] and [GFP-F2A18-CherryFP] polyproteins.
Western blotting data demonstrated that both MMP-2 and Twist expression were reduced by BITC and PEITC, in a dose-dependent manner.
Western blotting data demonstrated that the loss of CASP2 resulted in a higher extent of autophagy induction (as indicated by an increase in LC3-II) than the WT in the presence of oxidative stressors (Fig. 8A, i and ii) as well as HS (Fig. 8A, iii); whereas, no significant difference was observed between the WT and casp2 −/− in autophagy induced by COLC (Fig. 8A, iv).
Western blot results were consistent with RT PCR data, demonstrating that melatonin treatment increases Bim expression both at transcriptional and translational levels.
These bioinformatics studies were complemented by experimental data demonstrating that the protein is expressed by several strains based on western-blot carried out using an antibody raised against B. cenocepacia J2315 BCAL2958.
In this study, western blot and immunofluorescence data demonstrated that H3K9Ac was decreased and H3K9Me2 was increased globally upon MSC osteogenic differentiation (Figure 2A and B).
Taken together, Western blot and luciferase data demonstrate that PRKCD is a target of miR-26a.
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