Sentence examples for blot assays using a from inspiring English sources
We confirmed expression of the cFXN cassette under induction with increasing amounts of tetracycline by detection of the FLAG tag in western blot assays using a specific anti-FLAG antibody.
Mutp53 ubiquitination was determined by IP using DO-1 antibody (for mutp53) followed by Western blot assays using an anti-Ub antibody.
(D) The protein levels of AMFR and NOTCH1 were determined by Western blot assays using LoVo and HCT-116 cells transfected with miR-139-5p miR-139-5p miR-139-5pibitor (anti-mimic39-5p) miR-139-5p miR-139-5p miR-139-5pcontrol (NC or antinhibitor
To make this determination, we performed Western blot assays using different antibodies against viral proteins to detect viral-protein production in infected cells.
Initially performed western blot assays using different breast cancer cell line lysates provided positive signals with CB11 antibody, but not with others (data not shown).
A 30-kDa band, which migrates in three protein spots (pI~6.3, ~6.5, and ~6.7) with a different type and level of phosphorylation, was identified as TvLEGU-1 by one- and two-dimensional Western blot (WB) assays, using a protease-rich trichomonad extract and polyclonal antibodies produced against the recombinant TvLEGU-1 (anti-TvLEGU-1r).
The oligomeric structure was observed as a low mobility 250-kDa band in a Western blot assay using a specific anti-Cry1Ab antiserum.
Western blot assay using a Flag antibody indicated that all suppressors are expressed at a high level in the progenies from these crosses (Fig. S1).
To determine if the stained 140 kDa and 90 kDa bands correlated with direct dengue 3' SL RNA binding potential, a northwestern blot assay, using a radiolabeled dengue 3' SL RNA probe, was performed.
The expression levels of the p53 and p21 proteins were determined by western blot assay using a specific anti-p53 or anti-p21 antibody.
Enzymatic activity from the supernatant of the infected cells was demonstrated by dot blot assay using an antibody that recognizes the digested form of CSPG and was compared with the bacterial ChABC enzyme.
