Sentence examples for blot assay using a from inspiring English sources

Western blot assay using a Flag antibody indicated that all suppressors are expressed at a high level in the progenies from these crosses (Fig. S1).

The oligomeric structure was observed as a low mobility 250-kDa band in a Western blot assay using a specific anti-Cry1Ab antiserum.

To determine if the stained 140 kDa and 90 kDa bands correlated with direct dengue 3' SL RNA binding potential, a northwestern blot assay, using a radiolabeled dengue 3' SL RNA probe, was performed.

The expression levels of the p53 and p21 proteins were determined by western blot assay using a specific anti-p53 or anti-p21 antibody.

Enzymatic activity from the supernatant of the infected cells was demonstrated by dot blot assay using an antibody that recognizes the digested form of CSPG and was compared with the bacterial ChABC enzyme.

Flag-tagged β-catenin was immunoprecipitated and the acetylated β-catenin was detected by western blot assay using an anti-acetyl-lysine antibody.

Cell specificity was determined in cultures of primary microglia by Western blot assay using an antibody against OX-42 (a microglial marker) and qRT-PCR using primers for C1q (a microglial marker) and GFAP (an astrocyte marker).

The observed changes on POLDIP3 mRNA splicing were further confirmed at the protein level by performing western blot assay using an antibody that detects the two variants of this protein (Fig.  4C).

We confirmed expression of the cFXN cassette under induction with increasing amounts of tetracycline by detection of the FLAG tag in western blot assays using a specific anti-FLAG antibody.

Mucin in perfusates was quantified by periodic acid-Schiff's base dot-blot assay, using a kit (Sigma; catalog 395B).

The presence of the scaffoldin on the cell surface was assessed by dot-blot assay using a specific antibody against the CBM (Additional file 5: Figure S5).