Sentence examples for blot assay by using from inspiring English sources

The expressed fusion proteins were separated by SDS PAGE and confirmed via Western blot assay by using anti-VP3 monoclonal antibody (mAb) (TropBio, Australia) and anti-MBP mAb (New England Biolabs, USA).

Cell specificity was determined in cultures of primary microglia by Western blot assay using an antibody against OX-42 (a microglial marker) and qRT-PCR using primers for C1q (a microglial marker) and GFAP (an astrocyte marker).

FLIPr expression in S. aureus bacterial supernatant was confirmed by western blot assay using anti-FLIPr antibodies (Supplementary Fig. S2).

Enzymatic activity from the supernatant of the infected cells was demonstrated by dot blot assay using an antibody that recognizes the digested form of CSPG and was compared with the bacterial ChABC enzyme.

Densitometric analysis of endogenous LC3-I and -II detected by Western blot assay was performed using Multi Gauge V2.1 (Fuji Photo Film Company, Kanagawa, Japan).

The Mcl-1 ubiquitination level were analyzed by western blot assay using anti-HA antibody.

To this end, we carried out immunoprecipitation (IP) followed by western blot assay using nuclear extracts of senescent cells.

The expression of RBM25 and the mutant constructs was validated by Western blot assay using anti-FLAG antibodies.

Cell lysates made from T47D cells were subjected to immunoprecipitation with our antibodies and antibody-bound proteins were tested by western blot assay using CB11 antibody.

Flag-tagged β-catenin was immunoprecipitated and the acetylated β-catenin was detected by western blot assay using an anti-acetyl-lysine antibody.

The expression levels of the p53 and p21 proteins were determined by western blot assay using a specific anti-p53 or anti-p21 antibody.