Sentence examples for blot approach we confirmed from inspiring English sources

Using a combined IP and western blot approach, we confirmed that endogenous LRRK2 levels in control SHSY5Y cells were similar to those in the mouse cortex, but the levels in fibroblast and lymphoblast cultures were ∼7- and 100-fold higher than those seen in the brain.

By Western blot analysis, we confirmed a sufficient CaMKII take out in all experimental iDKO mice.

Using RT PCR and western blotting we confirmed that the transfected sequences were expressed.

First, we confirmed by Western blot that the Grp94−/− cells are devoid of GRP94 (Figure 6A).

We confirmed by Western blot analysis that aldolase A is expressed in uninduced NIH3T3-47/miR-122 cells.

We confirmed by western blot analysis that ZEB2 and B-catenin were upregulated.

Using immunohistochemistry, we confirmed the results of Western blot.

We confirmed the knockdown using PCR and Western blot after 24 96 h.

We confirmed depletion by both western blot analysis and quantitative RT PCR.

We confirmed SPG20 overexpression by western blot in both DLD1+13 and AF+13 cells.

Moreover, we confirmed these results using western blotting assay.