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Western blot analysis was utilised to determine the expression status of the MMTV-c- myc transgene in the mammary tumours and mammary gland tissue from parous study mice (c- myc transgene-negative mice were included as an assay negative control).
Thematic analysis was utilised by the qualitative studies [ 13, 30].
The Framework method of analysis was utilised [ 10].
Multivariate analysis was utilised to determine the most predictive factors affecting mortality within these patient groups.
qRT-PCR analysis was utilised for profile validation, miR and gene quantitation in patient SFs.
To determine the adjusted HRs (aHR) for the predictor variables, Cox multivariate regression analysis was utilised.
For all clinical outcomes, an intention-to-treat analysis was utilised.
The Preferred Reporting Items for Systematic Review and Meta-Analysis were utilised (Moher et al, 2009).
Dot blot and Western blot analysis were performed as described [46].
Western blotting procedure and reagents for western blot analysis were previously described [4].
Western blot analysis was performed to study the magnetic fraction.
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