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The results of Western Blot analysis were quantified by measuring the relative intensity compared to the control by using Kodak Molecular Imaging Software (Version 4.0.5, Eastman Kodak Company, Rochester, NY, USA) and represented in the relative intensities.
The densitometry data from the western blot analysis were quantified automatically using Multigauge Ver. 3.0 (Fujifilm, Tokyo Japan).
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The band intensity in Western blot analysis was quantified by ImageJ.
RNA expression was detected by Northern blot analysis and quantified using a Molecular Dynamics Storm 860 PhosphorImager/Fluorima. Protein expression detected by Western blot analysis was quantified using the ImageJ program.
Real-time RT-PCR and Western blot analysis were used to quantify Htt mRNA and protein levels, respectively, in human cell lines.
After northern blot analysis, data were quantified using ImageJ software and total RNA levels were corrected for 5S RNA.
For western blot analysis, proteins were quantified by the BCA protein assay (Pierce Chemical, Co., Rockford, Illinois, USA), transferred to nitrocellulose membranes (Schleicher & Schuell-Whatman, Maidstone, Kent, UK), and visualized by using Chemicon chemiluminescence reagents (Chemicon International, Inc., Temecula, California, USA).
Western blot analysis was conducted and quantified as described by Müller et al. [ 28].
Western blot analysis was employed to quantify total numbers of hippocampal NR2A and NR2B.
Western blot analysis was employed to quantify GFP expression.
Western blot analysis was also undertaken to quantify age-related changes in Aβ deposition in the mouse RPE/Bruch's membrane interface and in the retina.
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