The secondary antibodies HRP-conjugated goat anti-mouse IgG (H + L) (catalog number J092) for Western blot analysis were purchased from Transgen Biotech (China), and AlexaFluor 488-labeled goat anti-mouse IgG (H + L) (catalog number A0428) for immunofluorescence were purchased from Beyotime (China).
All of the antibodies used for Western blot analysis were purchased from Cell Signaling (Danvers, MA).
Various primary and secondary antibodies for Western blot analysis were purchased from Cell Signaling Technology, Inc. (Boston, MA, USA).
Phenylmethylsulfonyl fluoride (PMSF) and the components of the whole cell lysis buffer for western blot analysis were purchased from SIGMA (St . Louis MO, USA).
Various primary and secondary antibodies for Western blot analysis were purchased from Cell Signaling Technology, Inc. (Boston, MA, USA) and Santa Cruz Biotechnology Santaa Cruz, CA, USA).
Anti-mouse JNK, phospho-JNK, p38, phospho-p38, ERK, phospho-ERK, STAT1, phospho-STAT1, Tublin, and GAPDH for Western blot analysis were purchased from Cell Signaling Technology (Danvers, MA, USA).
Horseradish peroxidase substrate for Western blot analysis was purchased from NEN Life Science Products (Boston, MA).
Monoclonal rabbit anti-human actin for western blot analysis was purchased from Sigma-Aldrich Co. (St . Louis MO, USA).
Total RNA extraction and northern blot analysis were performed.
The intensities of LDHA bands from Western blot analysis were estimated by densitometric analysis.
